Avaliação de painel de marcadores prognósticos (Ki67, receptor de estrógeno, receptor de progesterona, HER2 e COX-2) em câncer de mama de cadelas e gatas com objetivo de melhor definir a estratégia terapêutica.
As amostras tumorais sujeitas aos testes de imuno-histoquímica para marcadores HER2, RE e RP devem ser fixados em formalina tamponada à 10% por pelo menos 6 horas, com no máximo 48 horas para HER2 e 72 horas para RE/RP.
O material requerido é o tecido fixado em formalina, de preferência tamponada, e incluído em parafina (bloco de parafina) com fragmento representando tumor mamário diagnosticado no exame histopatológico de rotina e cópia do relatório do patologista veterinário.
Prazo de Entrega do laudo 10 a 15 dias úteis
Bibliografia.
Zappulli et al. Surgical Pathology of tumors of domestic animals. Volume 2: Mammary tumors, edited by M. Kiupel, 2019.
Cassali GD, GE Lavalle, E Ferreira, A Estrela-Lima, AB De Nardi, C Ghever, RA Sobral, Renée L. Amorim, LO Oliveira, FAR Sueiro, HEO Beserra, AC Bertagnolli, CO Gamba, KA Damasceno, CB Campos, MR Araujo, LC Campos, LN Monteiro, FC Nunes, RS Horta, DC Reis, MCR Luvizotto, GM Magalhães, JB Raposo, AMR Ferreira, NM Tanaka, F Grandi, R Ubukata, K Batschinski, E M Terra, RCL Salvador, PC Jark, JER Delecrodi, NA Nascimento, DN Silva, LP Silva, KCRS Ferreira, MS Frehse, GW Santis, EO Silva, TN Guim, B Kerr, PP Cintra, FBF Silva, JS Leite, MF V Mello, MLG. Ferreira, H Fukumasu, BS. Salgado, Rafael Torres. Consensus for the Diagnosis, Prognosis and Treatment of Canine Mammary Tumors - 2013. Braz J Vet Pathol, 2014, 7(2), 38 – 69
Peña L, et al. Canine mammary tumors: a review and consensus of standard guidelines on epithelial and myoepithelial phenotype markers, HER2, and hormone receptor assessment using immunohistochemistry. Vet Pathol. 2014;51:127-45.
Nguyen F, et al. Canine invasive mammary carcinomas as models of human breast cancer. Part 1: natural history and prognostic factors. Breast Cancer Res Treat. 2017
Abadie J, et al. Canine invasive mammary carcinomas as models of human breast cancer. Part 2: immunophenotypes and prognostic significance. Breast Cancer Res Treat. 2017
Goldschmidt M, Pena L, Rasotto R, et al. Classification and grading of canine mammary tumors. Vet Pathol. 2011;48:117–131.
Estrógeno, Progesterona e HER2
Peña L, et al. Canine mammary tumors: a review and consensus of standard guidelines on epithelial and myoepithelial phenotype markers, HER2, and hormone receptor assessment using immunohistochemistry. Vet Pathol. 2014;51:127-45.
Geraldes M, et al. Immunohistochemical study of hormonal receptors and cell proliferation in normal canine mammary glands and spontaneous mammary tumours. Vet Rec. 20001;146:403-6.
Nieto A, et al. Immunohistologic detection of estrogen receptor alpha in canine mammary tumors: clinical and pathologic associations and prognostic significance. Vet Pathol. 2000;37:239–247
Geraldes M et al. Immunohistochemical study of hormonal receptors and cell proliferation in normal canine mammary glands and spontaneous mammary tumours. Vet Rec. 2001;146:403-6.
Araújo MR, et al. HER-2, EGFR, Cox-2 and Ki67 expression in lymph node metastasis of canine mammary carcinomas: Association with clinical-pathological parameters and overall survival. Res Vet Sci. 2016;106:121-30.
Ki67
Yang WY, et al. Proliferative activity, apoptosis and expression of oestrogen receptor and Bcl-2 oncoprotein in canine mammary gland tumours. J Comp Pathol. 2006 ;134:70-9.
Sarli G, et al. Prognostic value of histologic stage and proliferative activity in canine malignant mammary tumors. J Vet Diagn Invest. 2002;14:25-34.
Pena L, et al. Immunohistochemical detection of Ki-67 and PCNA in canine mammary tumors: relationship to clinical and pathologic variables. J Vet Diagn Invest. 1998;10:237–246.
Estrógeno e Progesterona
Peña L, et al. Canine mammary tumors: a review and consensus of standard guidelines on epithelial and myoepithelial phenotype markers, HER2, and hormone receptor assessment using immunohistochemistry. Vet Pathol. 2014;51:127-45.
Geraldes M, et al. Immunohistochemical study of hormonal receptors and cell proliferation in normal canine mammary glands and spontaneous mammary tumours. Vet Rec. 20001;146:403-6.
Nieto A, et al. Immunohistologic detection of estrogen receptor alpha in canine mammary tumors: clinical and pathologic associations and prognostic significance. Vet Pathol. 2000;37:239–247
Geraldes M et al. Immunohistochemical study of hormonal receptors and cell proliferation in normal canine mammary glands and spontaneous mammary tumours. Vet Rec. 2001;146:403-6.
COX-2
Clemente M, et al. Different role of COX-2 and angiogenesis in canine inflammatory and non-inflammatory mammary cancer. Vet J. 2013 Mar 11.
Millanta F, et al. COX-2 expression in canine and feline invasive mammary carcinomas: correlation with clinicopathological features and prognostic molecular markers. Breast Cancer Res Treat. 2006;98:115–120.
FELINOS
Cassali et al. Consenso para o diagnóstico, prognóstico e tratamento de
tumores mamários em felinos. Braz. J. Vet. Res. Anim. Sci., São Paulo, v. 55,
n. 2, p. 1-17, e135084, 2018.
De Campos et al. Evaluation of prognostic factors and survival rates in malignant feline mammary gland neoplasms. Journal of Feline Medicine and Surgery 2016,18,1003–1012.
Tumores mamários em cães machos
Bearss JJ, et al. Histologic, immunohistochemical, and clinical features of 27 mammary tumors in 18 male dogs. Vet Pathol. 2012;49:602-7.
Saba CF, et al. Mammary gland tumors in male dogs. J Vet Intern Med. 2007;21:1056–1059.
